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HomeFrequently Asked Questions
Quick answers to common questions about our services and processes.
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What does TC-treated mean in the product name?
+"TC-treated" means "TissueCultured" to treat the surface. PakGent helps cells adhere to the wall by improving the hydrophilicity of the surface of the corona discharge polystyrene(PS) to improve the adhesion of the proteins required for cell adhesion. -
Which surface should I use to culture the suspended cells?
+There is no need for cell adhesion in suspension cells, and generally, untreated surface culture consumables can be used. Suspension cells can also be cultured on theTC-treatedsurface, but some cells may adhere to the wall depending on the state and type of cells. -
What certificates do PakGent have for cell culture products? Is there amedical device registration?
+PakGent cell culture consumables are only used for scientific research, and thereisnoregistration certificate related to medical devices or drug packaging materials. Wecanprovide ISO quality certificate of production factory and quality certificate of eachlot of products.PakGent cell culture consumables are only used for scientific research, and thereisnoregistration certificate related to medical devices or drug packaging materials. Wecanprovide ISO quality certificate of production factory and quality certificate of eachlot of products.
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I want to coat myself with extracellular matrix. Which surface culture consumables should I choose?
+You can choose to self-coat the culture vessel on the untreated surface with the extracellular matrix. Of course, the utensils on the surface of the TC treatment can also be used in this case. -
How should the cell culture flask with plug cap be used? What is the difference between the use of the vent filter cap?
+The cell culture flask with the plug cap provided a closed cell culture environment. If thecell culture flask needed to be connected to the air in the incubator, the cap shouldbeloosened slightly after the culture flask was placed in the incubator to allowair entry. Similarly, when taking out, it is necessary to tighten the cap before taking out. Thevent filter cap itself has the function of air permeability, and the filter membrane canprevent microbial contamination. Therefore, when using, you can directly tighten the flaskcapintothe incubator, but you need to pay attention to not wet the filter membrane on theflaskcap,and the wet filter membrane can not ensure the gas through.
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What do I often hear about T25 and T175?
+Taking T25 as an example, it refers to a cell culture flask with a growth surface area of 25 cm2. According to the type of flask cap and the different culture surface, different products are corresponding to different product numbers. -
The cell culture flask/dish I purchased is not vacuum-packed, and the bag looks loose. Is there a quality problem?
+PakGent's cell-culture consumables were not vacuum-packed but were manually aspirated for ease of transport, so discrepancies may have occurred. In addition, the temperature and air pressure in different regions will also affect the tightness of the packaging. If thereis no air leakage in the packaging and the lid does not fall off from the product, it will not affect the sterile performance and product quality of the product, please feel free to use. Ifthe packaging is damaged or the lid is falling off the product, please take a photo and file acomplaint through your dealer, and please be careful to provide a photo of the outer caseofthe product. When you receive the goods, please carefully confirmwhether theouter caseis damaged or deformed. -
I used PakGent's culture flask/dish/plate, and the cells did not adhere to the wall. Is it a product quality problem?
+There are many factors leading to cell non-adherence, such as mediumcompositionandpHvalue, culture environment, digestion operation, cell type, cell passage and so on. If youusethe same product number for a long time to culture the same cell line and the cellsdonot adhere to the wall before, it is recommended that you record the batch number of theproduct used in the two cases in time, and repeat the parallel experiment, that is, usethecells from the same source on the two batches of consumables for culture (pay attentiontothe use of the same source of medium). Cultures were grown in the same incubator todetermine the main reason for nonadherent cells. -
The color of the culture flask/dish/plate I purchased this time is yellowthan that of the last time, is it the product quality problem?
+Normal. Due to the particularity of the sterilization process, the dose absorbed bytheproduct during the sterilization process is not consistent, which will lead to a slight differencein the color of the product. However, this does not affect the sterility of the product. All oursterilized products have been verified for sterilization, and we can provide a report. -
What certification has our pipette tips done?
+Our tips are Dnase, RNase, pyrogen, endotoxin, and ATP-free. Conforming to ISO13485: 2016quality control system.The materials conform to the requirements of USP Class VI. Irradiation sterilization does not leave toxic substances on the product like ethyleneoxidegas sterilization. Registered abroad as in vitro diagnostic consumables.
